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KMID : 0854720020220020436
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Korean Journal of Asthma, Allergy and Clinical Immunology
2002 Volume.22 No. 2 p.436 ~ p.445
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The production of interferon gamma by peripheral T lymphocytes in patients with allergic asthma upon stimulation with Der p2 antigen
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Abstract
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Background and Objectives: Recently, distinct cytokine-secreting subsets of CD8+ T cell, similar to their CD4+ Th1- and Th2-type counterparts, have been identified. In order to investigate the role of CD8+
T
cells
in the pathogenesis of allergic asthma, we determined the changes in the proportion of CD3, CD4+ and CD8+ T cells that expressed the IFN-γ by Dermatophagoides pteronyssinus antigen 2 (Der p2) activation.
Methods: Peripheral blood mononuclear cells (PBMCs) were obtained from mild persistent allergic asthmatic(n=12) and control subjects (n=8) and cultured with Der p2 for days, and then 12-0-tetracanoylphorbol-13-acetate and calcium ionophore
were
added 4 hours before staining. Surface CD3, CD4 or CD8 with intracytoplasmic IFN-γ was measured simultaneously by flow cytometry.
Results: Comparing asthmatics with control subjects, we found no differences in the percentage of CD3+, CD4+, or CD8+ T cells in PBMCs stimulated with or without Der p2. When PBMCs were cultured in media
only, the
proportion of cells producing the cytokines IFN-γ was similar in CD3+, CD4+ cells, but was significantly lower in CD8+ cells of the asthma group compared to the controls. Both CD4+ and CD8+
subsets
revealed a similar degree of reduction in IFN-γ positive cells in the asthma group compared with the controls by the stimulation of Der p2.
Conclusion: These findings suggest that CD8+ T cells may be important in the pathogenesis of asthmatic inflammation by modulating the production of IFN-γ.
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KEYWORD
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